Serial dilution11/23/2023 ![]() This dilution is typically falls within the linear range of the Bradford assay. Don’t forget to take this dilution into account when calculating the total protein concentration in the original, undiluted sample tube.Į3 1/2 – Elution 3 sample, 1/2 dilution. Don’t forget to take this dilution into account when calculating the total protein concentration in the original, undiluted sample tube.Į2 1/2 – Elution 2 sample, 1/2 dilution. Don’t forget to take this dilution into account when calculating the total protein concentration in the original, undiluted sample tube.Į1 1/2 – Elution 1 sample, 1/2 dilution. We recommend the following dilutions:įT 1/10 – Flow Through sample, 1/10 dilution. The dilutions depend on the experimental setup. Prior to setting up the 96-well plate, make any necessary dilutions for each protein purification sample tube.Please click this hyperlink to watch the multichannel pipette video This assay cannot be used to identify if the protein is DHFR-His-6x protein. Please note that the Bradford assay reports total protein concentration. You will be determining the total protein concentration in the flow through, wash, and elution fractions from your DHFR protein purification procedure. Do NOT work over your books! Always stay alert and UNDERSTAND what you are doing and WHY you are doing it! Please make sure that you work with liquids in your designated work surface. Multiskan Plate Reader with Accent Software.Diluted Bradford reagent (Bio-Rad Protein Assay Dye Reagent) – contains Coomassie Brilliant Blue G-250, phosphoric acid and methanol.Phosphate Buffered Saline (PBS) -137 mM NaCl, 2.7 mM KCl, 10 mM Na 2HPO 4, 2 mM KH 2PO 4. ![]() ![]() ![]() Sample fractions from protein purification lab. ![]()
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